Coverage / Read Count Calculator

Calculate how much sequencing you need to hit a target depth of coverage (or vice versa).

Instructions: set the read length/configuration and genome size, then select what you want to calculate.

Written by Stephen Turner, based on the Lander-Waterman formula, inspired by a similar calculator written by James Hadfield. Coverage is calculated as C=LN/G and reads as N=CG/L where C = Coverage (X), L = Read length (bp), G = Haploid genome size (bp), and N = Number of reads. Source code on GitHub.

Genome size

Pick a genome below or manually enter the haploid genome size in bp. You can use scientific notation (e.g., enter 3.2e9 for 3,200,000,000bp, or 3.2 Gb).